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Increased spontaneous reversion of certain frameshift mutations in DNA polymerase I deficient strains of Escherichia coli

✍ Scribed by Vaccaro, Karen K. ;Siegel, Eli C.

Publisher: Springer
Year: 1975
Tongue: English
Weight: 809 KB
Volume: 141
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00341803

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✦ Synopsis

A tenfold increase in the spontaneous reversion frequency of two of six lacZ frameshift mutations tested was observed in strains containing the following DNA polymerase I mutations--polA1, polA5, polA6, polAex1, res-3 and resA1. Reconstruction experiments indicated that this increase was not the result of a selective effect. Only a fourfold increase in frameshift mutations was found in strains containing a polA107 mutation. Both the polAex1 and polA107 mutations result in defective 5' to 3' exonuclease activity and do not affect polymerizing activity, but have different effects on frameshift mutation. A polA mutation on the chromosome induced frameshift mutations in a gene on an F episome. None of three auxotrophic mutations studied showed high frequency reversion in the presence of the polA1 or polA6 mutations.

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