Increased ribonucleotide reductase activity in hydroxyurea-resistant mosquito cells

✍ Scribed by Anna Gerenday; Karen M. Shih; Carter C. Herman; Ann M. Fallon

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 155 KB
• Volume: 34
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/(sici)1520-6327(1997)34:1<31::aid-arch3>3.0.co;2-z

✦ Synopsis

Hydroxyurea-resistant Aedes albopictus mosquito cells were selected by incremental exposure of unmutagenized cells to hydroxyurea concentrations ranging from 0.1 to 8 mM. Clonal populations that had become 40-fold more resistant to hydroxyurea than wild-type cells varied in morphology, and their growth rate decreased to a ~45 h doubling time, relative to an 18 h doubling time in unselected cells. At this level of resistance, the cells remained diploid, with a modal chromosome number of 6. When labelled with 35 S[methionine/cysteine], clone HU1062, which grew in the presence of 8 mM hydroxyurea, overproduced a labeled protein with the approximate size of the 45,000 dalton M2 subunit of ribonucleotide reductase. Consistent with this observation, ribonucleotide reductase activity in HU-1062 cells was approximately 10-fold higher than in wild-type control cells. This is the first example of an hydroxyurearesistant insect cell line.