Increased peripheral blood gamma delta T-cells in patients with lymphoid neoplasia: A diagnostic dilemma in flow cytometry
✍ Scribed by Julie McClanahan; Paula I. Fukushima; Maryalice Stetler-Stevenson
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 197 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
We have observed increased numbers of non-neoplastic ␥␦-T-cells in the peripheral blood of a series of patients with non-Hodgkin's lymphoma not of ␥␦-T-cell origin. The majority of normal ␥␦-T-cells are negative for surface CD4 and CD8 and a subpopulation does not express CD5, two immunophenotypic findings strongly suggestive of neoplasia in alpha beta T-cells. In addition, they express cytotoxic T-cell/Natural killer cell antigens. In this study, up to 22% of PBLs were CD4 and CD8 negative ␥␦-T-cells and up to 33% PBLs were CD5 negative ␥␦-T-cells. In addition, as high as 42% of PBLS were ␥␦-T-cells expressing cytotoxic T-cell/Natural killer cell antigens, suggestive of a large granular lymphoproliferative disorder. Failure to recognize that these are normal ␥␦-T-cells could lead to the erroneous diagnosis of peripheral blood involvement with a T-cell neoplasm, especially in the setting of a history of non-Hodgkin's lymphoma.