Increased messenger RNA levels for low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme a reductase in rat liver after long-term ethanol ingestion
✍ Scribed by Helmut K. Seitz; Bernhard Kuhn; Eberhard von Hodenberg; Walter Fiehn; Christian Conradt; Ulrich A. Simanowski
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 729 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Because long-term alcohol intake leads to severe alterations of cholesterol metabolism resulting in both elevated serum cholesterol levels and increased hepatic concentrations of cholesterol esters, we investigated the effect of long-term ethanol consumption on the hepatic messenger RNA (mRNA) content of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and low-density lipoprotein receptor, two major regulatory factors in cholesterol metabolism, and of apoprotein E. Twenty-four male Sprague-Dawley rats were pair-fed nutritionally adequate liquid diets containing 36% of total calories as either ethanol or isocaloric carbohydrates for 3 wk. In addition, the lipid content of the diets was varied, resulting in 35%, 17.5%, and 8.8% of total calories corresponding to a daily intake of cholesterol of between 1.2 and 6.3 mglkg body wt. Although increasing dietary cholesterol intake resulted in a sig nificant decrease of hepatic mRNA for low-density lipoprotein receptor and HMG-CoA reductase (p < 0.05), long-term ethanol consumption led to a significant increase of the mRNA for both proteins (p < 0.01), and this increase was predominantly obvious in animals fed a low-cholesterol diet. In contrast, mRNA content of apoprotein E was found to be significantly lower in livers from rats fed ethanol for a prolonged period of time as compared with controls (p < 0.011, and this effect was found to be still present, although less pronounced, after low cholesterol intake. These findings were paralleled by a significant increase in hepatic cholesterol esters (161 +-30 vs. 70 f 25 mg/100 gm; p < 0.01) but not free cholesterol and by elevated serum cholesterol levels (129 & 8 vs. 85 * 10