SH-SYSY cells differentiate into neuronal-like cells and express marker proteins like growth-associated protein (GAP-43) and neuropeptide tyrosine when treated with a low concentration (16 nM) of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in the presence of growth factors or serum.
Increased isoform-specific membrane translocation of conventional and novel protein kinase C in human neuroblastoma SH-SY5Y cells following prolonged hypoxia
✍ Scribed by Junfa Li; Yanming Qu; Pengyu Zu; Song Han; Ge Gao; Qunyuan Xu; Li Fang
- Book ID
- 113501667
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 601 KB
- Volume
- 1093
- Category
- Article
- ISSN
- 0006-8993
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The effects of phorbol esters on Ca 2 + channel currents in human neuroblastoma SH-SY5Y cells were studied using whole-cell patch-clamp recordings. Bath application of 12-O-tetradecanoylphorbol-13-acetate (TPA) or phorbol 12, 13-dibutyrate (PDBu; 100 nM to 1 #M), known activators of protein kinase C