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Increased ecto-metallopeptidase activity in cells undergoing apoptosis

✍ Scribed by Terrence J. Piva; Catherine M. Davern; Kari G. Francis; Grace M. Chojnowski; Paula M. Hall; Kay A.O. Ellem

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
265 KB
Volume
76
Category
Article
ISSN
0730-2312

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✦ Synopsis

Release from the cell surface of a variety of growth factors, cytokines, and proteases follows exposure to genetically stressful agents capable of inducing apoptosis and necrosis. Increased ectoprotease activity is responsible for their release. We show that increased activity of several metalloproteases on the HeLa cell surface occurs after stresses due to UVC, actinomycin D, cycloheximide, and cisplatinum, which induce the release of transforming growth factor-␣ (TGF␣) and other bioactive molecules. The ectoprotease activities increase preferentially on apoptotic cells, while little change occurs in viable cells. Gross decreases, except for the putative TGF␣ase activity, accompany necrosis. These changes may contribute to tissue repair and the absence of an inflammatory reaction to apoptotic cell death. They appear to be due to preferential enzyme activation or to retention by cells undergoing significant categorical decreases in protein content.

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