Increased circulating skin-homing cutaneous lymphocyte-associated antigen (CLA)+ type 2 cytokine-producing cells, and decreased CLA+ type 1 cytokine-producing cells in atopic dermatitis
✍ Scribed by Y. Teraki; T. Hotta; T. Shiohara
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 564 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0007-0963
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✦ Synopsis
Background:
Skin-homing t cells are characterized by expression of cutaneous lymphocyte-associated antigen (cla). few data are available on the frequency of circulating cla+ cytokine-producing t cells in atopic dermatitis (ad) patients.
Objectives:
We aimed to investigate cytokine synthesis capability vs. cla expression in phorbol myristate acetate and ionomycin-stimulated, secretion-inhibited peripheral blood t cells of ad patients compared with healthy subjects and psoriatic patients.
Methods:
Multiparameter flow cytometry was used.
Results:
The expression of cla among cd4+ t cells was significantly elevated in ad patients compared with healthy subjects and psoriatic patients, whereas there was no significant difference between each group in cla expression among cd8+ t cells. the frequency of interleukin (il)-4- and il-13-producing cells in ad patients was significantly higher than in healthy subjects (in both cd4+ and cd8+ t-cell subsets) and psoriatic patients (in cd4+ t cells). in contrast, the frequency of interferon (ifn)-gamma-producing cells was significantly reduced in ad patients, among both cd4+ and cd8+ t cells, compared with healthy subjects and psoriatic patients. moreover, in ad patients, the frequency of il-4- and il-13-producing cells was remarkably increased among the cla+ subset (in both cd4+ and cd8+ t cells), whereas the frequency of ifn-gamma-producing cells was decreased in the cla+ subset (in cd4+, but not in cd8+ t cells).
Conclusions:
These results provide evidence for the expansion of skin-homing type 2 cytokine-secreting t cells, associated with a reduction in skin-homing type 1 cytokine-producing t cells, in peripheral blood of ad patients.