✦ LIBER ✦
Increase of xanthan production by self-cloning of a 3.0-kbEcoRI-KpnI chromosomal fragment inXanthomonas campestris
✍ Scribed by Chi-Liang Wei; Ka-Tim Choy; Nien-Tsung Lin; Yi-Hsiung Tseng
- Book ID
- 104633811
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 315 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
G76E is a non-mucoid mutant derived from Xanthomonas campestris pv. campestris 17 by Tn mutagenesis. A 3.0-kb EcoRI-KpnI fragment, cloned from a genomic bank of Xcl7, was able to complement G76E and caused an increase ofxanthan production by ca. 46% over Xcl7. This 3.0-kb EcoRI-KpnI fragment insert was different from the two previously cloned EcoRI fragments from Xcl7 that also increased xanthan production.