Increase in pituitary melanocyte-stimulating hormone activity of genetically obese (ob/ob) mice
โ Scribed by Margaret H. Peaslee; Beatriz Moisset; H. Shibuya; Candace B. Pert
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 234 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1420-682X
No coin nor oath required. For personal study only.
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The insulin, glucagon, somatostatin and pancreatic polypeptide content of acid-ethanol extracts of pancreas from lean and obese (ob/ob) mice of various ages was determined by radioimmunoassay. Rat and mouse pancreatic polypeptide react weakly with antibodies to avian, bovine and canine pancreatic po
Adenylate cyclase activity was determined using a purified plasma membrane fraction prepared from isolated adipocytes by a Percoll gradient centrifugation procedure. The activation of adenylate cyclase by isoprenaline was reduced by approximately 50% in membranes from obese mice, although no reducti