Incorporation of Tryptophan Analogues into Staphylococcal Nuclease: Stability toward Thermal and Guanidine-HCl Induced Unfolding †

Biosynthetic incorporation of tryptophan

Biosynthetic incorporation of tryptophan analogues into staphylococcal nuclease: Effect of 5-hydroxytryptophan and 7-azatryptophan on structure and stability

✍ Cing-Yuen Wong; Maurice R. Eftink 📂 Article 📅 2008 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 961 KB

5-Hydroxytryptophan (5HW) and 7-azatryptophan (7AW) are analogue of tryptophan that potentially can be incorporated biosynthetically into proteins and used as spectroscopic probes for studying protein-DNA and protein-protein complexes. The utility of these probes will depend on the extent to which t