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Incorporation of tritium into irradiated nucleotides

✍ Scribed by T. Kunieda; L. Grossman; B. Witkop


Book ID
118850814
Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
167 KB
Volume
33
Category
Article
ISSN
0006-291X

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We have used classical hot-atom chemistry to put tritium atoms inside fullerene molecules. The tritium is generated in a nuclear reactor with the reaction 6Li(n, a)3H. The hot tritium atom slows down and can end up being thermalized inside a fullerene where it is trapped. The irradiated sample is di

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An exchange method is described for producing tritium-labeled native DNA in vitro with minimal physical damage to the DNA. Tritium-labeled calf thymus DNA prepared in this way has a specific activity of about 100 pCi/mmole of nucleotide (i.e., about 2 x 108 dpm/mmole). Sedimentation velocity a t neu