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Incorporation of spin-labelled amino acids into proteins

✍ Scribed by Christian F. W. Becker; Kester Lausecker; Mária Balog; Tamás Kálai; Kálmán Hideg; Heinz-Jürgen Steinhoff; Martin Engelhard

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
191 KB
Volume
43
Category
Article
ISSN
0749-1581

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✦ Synopsis

The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins.

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