✦ LIBER ✦

Incorporation of radioactive label into nucleic acids of compatible and incompatible pollen tubes ofLilium longiflorumThunb.

✍ Scribed by Robert J. Campbell; Peter D. Ascher

Book ID: 104691529
Publisher: Springer
Year: 1975
Tongue: English
Weight: 568 KB
Volume: 46
Category: Article
ISSN: 0040-5752
DOI: 10.1007/bf00264869

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✦ Synopsis

Stylar canal cells of Lilium longiflorum were labeled before pollination with 5-(3)H-uridine or 5-(3)H-orotic acid dissolved in water, or the stylar canal was filled 6 hr after pollination with stigmatic exudate supplemented with 5-(3)H-uridine, 2-(14)C-uridine or H3 (32)PO4. Nucleic acids extracted from compatible and incompatible pollen tubes grown in these styles were separated by methylated albumin kieselguhr column chromatography. Label occurred in all portions of the RNA profile, but no label appeared in the DNA peak. Using 5-(3)H-uridine, pollen tubes of different genotype had different labeling patterns.

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