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Incorporation of inositol hexaphosphate into red blood cells mediated by dimethyl sulfoxide

โœ Scribed by R.S. Franco; M. Weiner; K. Wagner; O.J. Martelo

Book ID
118941913
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
340 KB
Volume
32
Category
Article
ISSN
0024-3205

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Incorporation of inositol hexaphosphate
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Fluid charged lipid vesicles loaded with an inositol hexaphosphate solution were used to transport this allosteric effector into human intact red blood cells. Rate and extent of uptake of the vesicles and of the effector by the red blood cells were measured as changes in the O2 half-saturation press

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Incorporation of IHP into red cells decreases oxygen affinity as a result of the binding of this compound to the 2,3-DPG site of hemoglobin. This investigation describes a continuous-flow method which utilizes the osmotic pulse technique to transport I H P into RBC. Using this procedure, it is possi