Incorporation of carbon nanotubes in a silica HPLC column to enhance the chromatographic separation of peptides: Theoretical and practical aspects
✍ Scribed by Claire André; Rania Aljhani; Tijani Gharbi; Yves C. Guillaume
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 259 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The retention mechanism of a series of peptides on a single‐wall carbon nanotube (SWCNT) stationary phase inside an HPLC column was investigated over a wide range of mobile phase compositions. While the similar size C18 column exhibited an efficiency of 11.5 μm, the SWCNT column increased the efficiency, i.e. 7.10 μm at a flow rate of 0.8 mL/min, and significantly affected the separation quality of the peptides. The values of enthalpy (Δ__H__) and entropy (Δ__S__^*^) of transfer of the peptides from the mobile to the SWCNT stationary phase were determined. The method studied each factor, i.e. ACN fraction x in the ACN/water mixture and column temperature. The changes in retention factor, Δ__H__ and Δ__S__^*^ as a function of the ACN fraction in the mobile phase were examined. These variations are explained using the organization of ACN in clusters in the ACN/water mixture and on the steric and electronic forces implied in the retention process. The information obtained in this work makes this SWCNT stationary phase useful for peptide research and demonstrated the role of ACN to improve the separation quality.