Incorporation of anthraquinonyl group into λ-Cro repressor protein for strand- and position-specific photocleavage of double-stranded DNA
✍ Scribed by Hiroshi Sasaki; Kazumasa Ikeda; Maya Suzuki; Keiko Ninomiya; Masahiko Sisido
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2004
- Tongue
- English
- Weight
- 185 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
λ‐Cro repressor protein incorporated with a 2‐anthraquinonylalanine (anqAla) at the 64th position was chemically synthesized by solid‐phase method. The 64th position was selected according to previous information on various mutants of Cro incorporated with a single anqAla unit, that were synthesized through an Escherichia coli in vitro protein‐synthesizing system. The 64anqAla mutant bound to a dsDNA of consensus operator sequence and underwent strand‐ and position‐specific photocleavage of the dsDNA at the GG sequence after treatment with piperidine. The mutant also underwent position‐specific self‐photoscission. The self‐photoscission was retarded in the presence of the dsDNA. © 2004 Wiley Periodicals, Inc. Biopolymers (Pept Sci) , 2004