Incorporation of A Phosphonium Analogue of Choline Into The Rat Brain as Measured by Magnetic Resonance Spectroscopy
✍ Scribed by Jilma V. Jiménez; Todd L. Richards; Aaron C. Heide; John R. Grierson; Eric G. Shankland
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 734 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
A clear understanding of choline metabolism is important in our goal to modify demyelination and remyelination in multiple sclerosis. To develop a technique capable of measuring metabolic changes in the brain, we have studied the incorporation of a phosphonium analogue of choline (P‐choline) in tissue extracts of rats. After feeding adult rats a choline‐deficient diet supplemented with P‐choline, the analogue was not detectable by in vivo volume‐localized ^1^H spectroscopy. However, in vitro ^31^P measurements of brain extracts revealed an 11 % incorporation of P‐choline into phosphatidylcholine. We report that P‐choline incorporates preferentially into the lipid pool over the lipid precursor pool and we provide evidence that the choline peak resolved by in vivo ^1^H spectroscopy is only composed of small molecular weight choline‐containing compounds.