Incorporation of W8, during cometaholic degradation of 3-chloroaniline by Rhodococcus sp. An 117 1). J A X K E ~) , B. 1'. BASKYNOV~), &I. Yv. N T ~C F H T ~O V A ~) , .A. h1. YAK^ K ~) and TA. A. (:o1,0v~eva2) (Eingegtmgen nrn 28. I). 1983)
Studies on the effect of' different oxygen supply on cometabolic degradation of 3-chloroaniline by resting aniline-grown cells of Rhodococcus sp. An 117 revealcd that atmospheric oxygen is required for the enzymatic rcwt'ion initiat'ing this degradative process which under "normal" conditions ( i . e . cell incabation in air atmosphere) is chara'ctcrized by a transient accumulittion of 4-ohlorocntcchol. I n oxygen atmosphere, however, no appreciable amounts of' 4-chlorocatechol were detected in the incabation medium, probably because of an activation of thr ring-cleavage reaction in An 117 cclls. Instead of, a. nonchlorimted compound accurnulat,ed which wss identified a.s thc ;)-lact'one of @-hydroxymuconic a'cid (y-carboxymethylene-A@-butenolide). The results of cxperiments wit,h I%, as well as H,I*O indicate that format'ion of the latter intermediate was duc to incorporation of four at.oms of a,tmospheric oxygen and t,hnt the initid conversion of monoclilorinnt,ed anilines into chlorocatechols by Rhodococcus sp. An 117 is a dioxygenase-mediated reaction.
Chlorinated anilines are well-established intermediates in degradation of various phenylrirea, acylanilide, and phcnylcarbamatc herbicides. With a few exceptions (ZEYER and KEARNEY 1982), they do not support microbial grom th, hut are subjected to cornetabolic degradation by certain aniline-degrading hactcrial cult tires supplicd with suitable additional carhon sources (REBER et nl.