Incorporation de l'antigène tumoral de transplantation spécifique du SV40 (TSTA-SV40) dans le virus de la stomatite vésiculleuse cultivé en cellules de hamster transformées par le SV40

✍ Scribed by S. Ansel

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 789 KB
• Volume: 13
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910130606

✦ Synopsis

Abstract

Highly purified and inactivated VSV (VSV/TSV‐5 C1.2) grown in SV40 transformed hamster cells, TSV‐5 C1.2, induces in hamster a transplantation immunity against host‐tumor cells. Immunization with VSV/TSV‐5 C1.2 gave more than a 10‐fold protection against TSV‐5 C1.2 tumor cells. The transplantation immunity confered by the immunization is specific for tumor cells which possess SV40‐TSTA. VSV/TSV‐5 C1.2 protects hamsters against another SV40‐transformed cell line, EHSVi‐C1.1. In contrast, immunization with VSV/EHB, a purified preparation of VSV grown in spontaneously transformed hamster cells, EHB, gave no protection to TSV‐5 C1.2 cell challenge. It was concluded that the presence of SV40‐TSTA activity in VSV/TSV‐5 C1.2 virions was due to incorporation of this tumor cell antigen in the viral envelope. The existence of cell antigens in VSV/TSV‐5 C1.2 and VSV/EHB preparations was also shown in vitro by Cr^51^ release assay. The anti‐VSV/TSV‐5 C1.2 rabbit serum reacted specifically with SV40‐transformed cells, TSV‐5 C1.2 and EHSVi‐C1.1.