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Incorporation and Asymmetric Orientation of Glycophorin in Reconstituted Protein-Containing Vesicles

✍ Scribed by Everardus J. J. van ZOELEN; Arie J. VERKLEIJ; Robert F. A. ZWAAL; Laurens L. M. van DEENEN


Book ID
115116698
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
837 KB
Volume
86
Category
Article
ISSN
1432-1327

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Reconstitution and electron paramagnetic
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Glycophorin A containing dimyristoylphosphatidylcholine vesicles has been reconstituted by reverse-phase evaporation, detergent dialysis, freeze and thaw cycles as well as by rehydration of a lipid-protein-film. The availability of each method has been tested. Reproducible results with a highly puri