Sultone-Assisted Inactivation of α-Chymo
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T.C. Liang; V. Caluya
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Article
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1994
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Elsevier Science
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English
⚖ 395 KB
Sultone and phenylmethanesulfonyl fluoride (PMSF) form sulfonyl-enzymes with serine proteases in a similar fashion. However, the sultone-derived sulfonyl-enzyme is readily hydrolyzed to regenerate active enzyme, while that derived from PMSF is not. Thus, sultone is regarded as a substrate rather tha