The glutamine synthetase was inactivated in vivo by incubation of the cell suspension with 2 β’ 10 -a ~ NH~ + for 2 min. The inactivated glutamine synthetase was extracted from the cells and purified 20-fold. Incubation of the purified glutamine synthetase with phosphodiesterase regenerated the bios
Inactivation of Escherichia coli glutamine synthetase by thiourea trioxide
β Scribed by Carolyn L. DiIanni; John A. Colanduoni; Joseph J. Villafranca
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 488 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0045-2068
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Under physiological conditions, the activity of the glutamine synthetase in gram-negative bacteria is inversely proportional to the number of its subunits that are adenylylated [Kingdon, H. S., Shapiro, B. M., and Stadtman, E. R., (1%7), Proc. Nat. Acad. Sci. U. S. A. 58, 1703-17101. Six different e
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