In vivo13C NMR analysis of acetate metabolism inThiobacillus versutusunder denitrifying conditions

✍ Scribed by P. A. M. Claassen; C. Dijkema; J. Visser; A. J. B. Zehnder

• Book ID: 104759208
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 630 KB
• Volume: 146
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00403221

✦ Synopsis

The disappearance of 2-13C-acetate and the subsequent incorporation of label into cellular metabolites were followed in denitrifying cells of Thiobacillus versutus by 3C NMR spectroscopy. In cells grown under acetate-limitation, the specific rate of consumption was idependent of the density of the celt suspension. An isotopic steady state was reached within 30 min if sufficient substrate was added to the cell suspension. In cells grown under nitrate-limitation, the consumption of 2-13C-acetate proceeded at a significantly lower rate. The decrease and final disappearance of 2-~3C-acetate were accompanied by incorporation of 13C into glutamate, glutamine, and by the release of labeled HCO~ and CO/. The appearance of a broad resonance being the methyl endgroup of poly-3-hydroxybutyrate (PHB) was indicative for PHB mobilization during the incubation. The sequence of label incorporation and the distribution among the various carbon nuclei were consistent with the operation of the tricarboxylic acid cycle.