In vivo utilization of N-(phosphonomethyl)-anilines and related substances by Pseudomonas spec. GS

✍ Scribed by Birgit Albrecht; Rosemarie Weidhase; Manfred Stock; Prof. Dr. Reinhard Albrecht Weidhase

• Book ID: 102388584
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 462 KB
• Volume: 31
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620310602

✦ Synopsis

Utilization of various phosphonates as source for phosphorus by the glyphosate degrading strain Pseudomonas spec. GS was investigated. Metabolites of phosphonate degradation were characterized indicating the cleavage of the C-P-bound as primary step of breakdown. The phosphonate N-(phosphonomethyl)-4'-nitroazobenzene-4-amine (azophon) was characterized as a suitable substrate for detection of C-P-bond splitting activity in vivo. Pseudomonas cells permeabilized by toluene treatment were also capable of phosphonate degradation whereas no in vitro activity of a putative C-P-bond cleaving enzyme was detectable after cell disruption.