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In vivo transcription initiation and termination sites of an α-amylase gene from Bacillus amyloliquefaciens cloned in Bacillus subtilis

✍ Scribed by Lehtovaara, Päivi; Ulmanen, Ismo; Palva, Ilkka


Book ID
123003982
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
532 KB
Volume
30
Category
Article
ISSN
0378-1119

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Following shotgun cloning of EcoRI fragments of Bacillus subtilis DNA in pBR322, a hybrid plasmid pUL710 was isolated which complements argC but no other auxotrophs of E. coli K12. Restriction mapping, Southern blotting and other evidence suggest that pUL710 carries an insert of 1.6 kbp, and derives