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In vivo selection of single-chain antibodies using a yeast two-hybrid system

✍ Scribed by Antje Pörtner-Taliana; Marijane Russell; Karen J Froning; Paul R Budworth; John D Comiskey; James P Hoeffler

Book ID
114050513
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
973 KB
Volume
238
Category
Article
ISSN
0022-1759

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We describe a simple and efficient one-step method to make cDNA libraries using homologous recombination in yeast. cDNA from any source, together with a linear vector, is used to transform yeast. Through homologous recombination and gap repair, the cDNA is unidirectionally incorporated into the yeas