## Abstract __Ex vivo__ selection of transduced hematopoietic stem cells (HSC) with drugβresistance genes offers the possibility to enrich transduced cells prior to engraftment, toward increased reconstitution in transplant recipients. We evaluated the potential of highly methotrexate (MTX)βresista
β¦ LIBER β¦
In vivo selection of human embryonic stem cell-derived cells expressing methotrexate-resistant dihydrofolate reductase
β Scribed by Gori, J L; Tian, X; Swanson, D; Gunther, R; Shultz, L D; McIvor, R S; Kaufman, D S
- Book ID
- 109855236
- Publisher
- Nature Publishing Group
- Year
- 2009
- Tongue
- English
- Weight
- 966 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0969-7128
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## Abstract In this study a three step culture system, 2Dβ3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Fiveβdayβold embryoid bodies were plated for chondrogenic induction for 27 days (