In vivo radioprotective effects of basic fibroblast growth factor (FGF2) in total body irradiated C3H/HeNCr mice
✍ Scribed by Ivan Ding; Marjaneh Moini; Nobuyoki Aotsuka; Martin J. Thoolen; Thomas M. Reilly; Paul Okunieff
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 856 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1065-7541
No coin nor oath required. For personal study only.
✦ Synopsis
Fibroblast growth factor (FGF,) was recently found to radioprotect endothelial cells. We examined its in vivo radiomodifying effects on bone marrow in total body irradiated (TBI) mice. Female C3H/HeNCr mice were given FGFz intravenously. Total FGF, per mouse ranged from 1 to 24 pg, delivered 24 and 4 hr before TBI or 24 and 48 hr after irradiation. Histologic examinations were performed of the marrow and other organs, and serum cytokine levels of tumor necrosis factor-a (TNF-a), interleukin-3 (IL-3), and granulocyte-macrophage colony stimulating factor (GM-CSF) were determined from FGF,-or saline-treated mice with an enzyme-linked immunosorbent assay (ELISA) technique. FGF, given pre-TBI significantly improved the survival of lethally irradiated mice. FGF, radioprotection was dose-dependent and was maximized at a dose of 6 pg/ mouse. The radiomodification was 1.16 f 0.04 ( k l S.D.) with an increase of LDSono from 771 & 10 to 891 f 28 cGy. Some radiomodification was even observed when FGFz was given 24 hr after irradiation (P < 0.05). FGF, radioprotected mice through a mechanism that improved recovery of bone marrow hematopoietic cells. The absence of abnormal IL-3, GM-CSF, or TNF-a blood levels favors a direct proliferative effect on hematopoietic tissue rather than an indirect induction of cytokines. Other possible mechanisms of FGF, action are discussed.