In vivo protein-DNA interactions at the c-jun promoter in quiescent and serum-stimulated fibroblasts

✍ Scribed by Dieter Rozek; Gerd P. Pfeifer

• Book ID: 102878863
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 763 KB
• Volume: 57
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240570313

✦ Synopsis

c-Jun is an important component in the regulation of cell proliferation. As a member of the early response gene family, c-jun is induced within minutes in the presence of mitogenic agents such as serum growth factors. Using in vivo footprinting, we have analyzed protein-DNA interactions at the c-jun promoter in human fibroblasts subjected to growth arrest and serum stimulation. We located seven footprints upstream of the transcription initiation site. Protein-DNA interactions were detected at two AP-I-like sequences, a CCAAT box, an SP-1 sequence, an NF-jun sequence, a putative RSRF (related to serum response factor) binding site, and a sequence bound by an unknown factor. All of these binding sites were occupied in serum-starved cells, and no additional protein-DNA interactions were detected upon serum stimulation. Evidence from this study supports a model in which expression of the c-jun gene is mediated by phosphorylation events taking place on the transactivation domains of promoter-bound transcriptional activators. D 1995 wiley-Liss, Inc.