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In vivo persistence of a HIV-1-encoded HLA-B27-restricted cytotoxic T lymphocyte epitope despite specific in vitro reactivity

✍ Scribed by Andreas Meyerhans; Gilles Dadaglio; Jean-Pierre Vartanian; Pierre Langlade-Demoyen; Ronald Frank; Birgitta Åjö; Fernando Plata; Simon Wain-Hobson

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
433 KB
Volume
21
Category
Article
ISSN
0014-2980

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✦ Synopsis

In vivo persistence of a HIV-1-encoded HLA-B27-restricted cytotoxic T lymphocyte epitope despite specific in vitro reactivity*

A large number of human immunodeficiency virus type 1 (HIV-1) specific HLA-restricted cytotoxicTcel1 (CTL) epitopes have been mapped, including an HLA-B27-restricted immunodominant epitope within p25gag. Accordingly, this segment of the HIV-1 provirus was amplified by the polymerase chain reaction from DNA derived from fresh uncultured peripheral blood mononuclear cells (PBMC) of four HLA-B27 HIV-l-infected individuals. In all cases the majority of infected PBMC bore sequences encoding the HLA-B27-restricted peptide. CTL escape mutants had not accumulated in vivo 8 and 14months later despite demonstrable CTL activity in vitro. These data emphasize the importance of silently infected lymphocytes in evading immune surveillance.

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