In vivo performance of a sol–gel glass-coated collagen

✍ Scribed by Ângela Leão Andrade; Sílvia Passos Andrade; Rosana Zacarias Domingues

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 354 KB
• Volume: 79B
• Category: Article
• ISSN: 1552-4973
• DOI: 10.1002/jbm.b.30521

✦ Synopsis

Abstract

Synthetic bioactive materials offer possibilities to repair large tissue defects. It is well known that bioactivity, angiogenesis, and inflammation are key events in implant incorporation. Using glass‐coated and glass‐free collagen as potential bone graft substitutes, we carried out in vitro bioactivity and an in vivo angiogenesis and inflammation studies. The in vitro study showed bioactivity when the glass‐coated samples were left in SBF for 5 days. This was confirmed by FTIR results, which presented PO vibration bands characteristic of hydroxyapatite close to 1060 cm^−1^ and 600 cm^−1^. The in vivo response was evaluated following subcutaneous implantation of the biomaterial in the mouse dorsa. Angiogenesis, as determined by hemoglobin content extracted from implants 7 and 14 days after implantation, increased progressively in both glass‐coated and glass‐free collagen implants. However, vascularization was higher in the glass‐coated collagen implants 14 days after implantation (μg Hb per mg wet tissue 6.0 ± 0.3) compared with the glass‐free group (1.6 ± 0.1). The inflammatory process, determined by the levels of myeloperoxidase and N‐acetylglucosaminidase, was similar for both implants. This study shows that glass‐coated collagen implants hold osteogenic and angiogenic potential and may be used in clinical conditions requiring improvement of these biological processes. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006