In vivo neurogenesis is inhibited by neutralizing antibodies to basic fibroblast growth factor

✍ Scribed by Tao, Y. ;Black, I. B. ;DiCicco-Bloom, E.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 88 KB
• Volume: 33
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199709)33:3<289::aid-neu7>3.0.co;2-y

✦ Synopsis

While extracellular growth factors pocampus was decreased by 53% and 63%, respecgovern neuronal precursor mitosis in culture, little is tively, suggesting that endogenous bFGF was involved known about their roles in regulating neurogenesis in vivo. Previously, we reported that subcutaneously in brain development. To define effects on neurogenadministered basic fibroblast growth factor (bFGF) esis specifically, granule cell precursors were isolated promoted neuroblast proliferation in P1 rat brain, in after antibody treatment. [ 3 H]dT incorporation in regions in which bFGF and FGF receptors are exgranule precursors was decreased by 50%, indicating pressed during development. To define the role of enthat the neutralizing antibody inhibited neuroblast dogenous bFGF in neurogenesis, we employed a neuproliferation in vivo. In contrast, no reduction was tralizing monoclonal antibody to the factor. In culture, observed using nonneutralizing or the heat-inacti-bFGF-induced granule precursor proliferation was vated antibodies. The inhibition of precursor proliferprogressively inhibited by increasing concentrations ation following immunoneutralization of bFGF in vivo of antibody. In contrast, heat-inactivated or nonneusuggests that the endogenous factor normally regutralizing anti-bFGF antibodies were ineffective. The lates brain neurogenesis.