In vivo magnetic resonance imaging of iron oxide–labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: Detection and monitoring at 3T
✍ Scribed by Harald Ittrich; Claudia Lange; Florian Tögel; Axel R. Zander; Hannes Dahnke; Christof Westenfelder; Gerhard Adam; Claus Nolte-Ernsting
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 975 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1053-1807
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Purpose
To evaluate MRI for a qualitative and quantitative in vivo tracking of intraaortal injected iron oxide–labeled mesenchymal stem cells (MSC) into rats with acute kidney injury (AKI).
Materials and Methods
In vitro MRI and R~2~* measurement of nonlabeled and superparamagnetic iron oxide (SPIO)‐labeled MSC (MSC~SPIO~) was performed in correlation to cellular iron content and cytological examination (Prussian blue, electron microscopy). In vivo MRI and R~2~* evaluation were performed before and after ischemic/reperfusion AKI (N = 14) and intraaortal injection of 1.5 × 10^6^ MSC~SPIO~ (N = 7), fetal calf serum (FCS) (medium, N = 6), and SPIO alone (N = 1) up to 14 days using a clinical 3T scanner. Signal to noise ratios (SNR), R~2~* of kidneys, liver, spleen, and bone marrow, renal function (creatinine [CREA], blood urea nitrogen [BUN]), and kidney volume were measured and tested for statistical significance (Student's t‐test, P < 0.05) in comparison histology (hematoxylin and eosin [H&E], Prussian blue, periodic acid‐Schiff [PAS], CD68).
Results
In vitro, MSC~SPIO~ showed a reduction of SNR and T~2~* with R~2~* ≈ number of MSC~SPIO~ (R^2^ = 0.98). In vivo MSC~SPIO~ administration resulted in a SNR decrease (35 ± 15%) and R~2~* increase (101 ± 18.3%) in renal cortex caused by MSC~SPIO~ accumulation in contrast to control animals (P < 0.01). Liver, spleen, and bone marrow (MSC~SPIO~) showed a delayed SNR decline/R~2~* increase (P < 0.05) resulting from MSC~SPIO~ migration. The increase of kidney volume and the decrease in renal function (P < 0.05) was reduced in MSC‐treated animals.
Conclusion
Qualitative and quantitative in vivo cell‐tracking and monitoring of organ distribution of intraaortal injected MSC~SPIO~ in AKI is feasible in MRI at 3T. J. Magn. Reson. Imaging 2007;25:1179–1191. © 2007 Wiley‐Liss, Inc.