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In vivo laser scanning microscopic investigation of the decontamination of hazardous substances from the human skin

✍ Scribed by J. Lademann; A. Patzelt; S. Schanzer; H. Richter; I. Gross; K.H. Menting; L. Frazier; W. Sterry; C. Antoniou

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 191 KB
Volume: 7
Category: Article
ISSN: 1612-2011
DOI: 10.1002/lapl.201010068

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✦ Synopsis

The stimulation of the penetration of topically applied substances into the skin is a topic of intensive dermatological and pharmacological research. In this context, it was found that in addition to the intercellular penetration, the follicular penetration also represents an efficient penetration pathway. The hair follicles act as a long-term reservoir for topically applied substances. They are surrounded by all important target structures, such as blood capillaries, stem and dendritic cells. Therefore, the hair follicles, as well as the skin, need to be protected from hazardous substances. The traditional method of decontamination after respective accidental contacts consists of an intensive washing of the skin. However, during this mechanical procedure, the substances can be pushed even deeper into the hair follicles.

In the present study, absorbent materials were applied to remove a fluorescent model substance from the skin without inducing mechanical stress. The results were compared to the decontamination effects obtained by intensive washing. Investigations were performed by means of in vivo laser scanning microscopy (LSM). The comparison revealed that decontamination with absorbent materials is more effective than decontamination with washing processes. 50 μm LSM image of the distribution of the fluorescent dye after application and penetration; the dye is located on the skin surface and in the first layers of the corneocytes

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