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In vivo labeling of fission yeast DNA with thymidine and thymidine analogs

โœ Scribed by Sasirekha Sivakumar; Mary Porter-Goff; Prasanta K. Patel; Kristen Benoit; Nicholas Rhind


Book ID
116986447
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
712 KB
Volume
33
Category
Article
ISSN
1046-2023

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Inhibition of thymidine phosphorylase in
โœ Womack, John E. ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 350 KB

Uridine blocks the in vivo conversion of thymine to thymidine in Escherichia coli, thus, one can change DNA labels by labelling first with a thymine label (e.g. 14C) and then, at the time of the change, adding 50 microgram uridine per ml and thymidine (e.g. 3H). The cells immediately start using the