In vivo imaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography

✍ Scribed by Molly Subhash Hrebesh; Razvan Dabu; Manabu Sato

• Book ID: 103874035
• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 862 KB
• Volume: 282
• Category: Article
• ISSN: 0030-4018
• DOI: 10.1016/j.optcom.2008.10.070

✦ Synopsis

We demonstrate the feasibility of a compact single-shot full-field time domain optical coherence tomography (OCT) for imaging dynamic biological sample in real-time. The system is based on a Linnik type polarization Michelson interferometer and a four-quadrature phase-stepper optics, which can simultaneously capture four quadraturely phase-stepped interferograms on a single CCD. Using a superluminescent diode as light source with center wavelength of 842 nm and spectral width of 16.2 nm, the system yields an axial resolution of 19.8 lm, and covers a field of view of 280 Â 320 lm 2 (220 Â 250 pixels) with a transverse resolution of 4.4 lm by using a 10Â microscope objective (0.3 NA). Three-dimensional OCT images of biological samples such as an onion slice and a diaptomus were obtained without any image averaging or pixel binning. In addition, in vivo depth resolved dynamic imaging was demonstrated to show the beating internal structure of a diaptomus with a fame rate of 5 fps.