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In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype

✍ Scribed by Herrmann, Heidrun ;Janke, Dieter ;Krejsa, Synnöve ;Roy, Michael

Publisher: Springer
Year: 1988
Tongue: English
Weight: 681 KB
Volume: 214
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00340199

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✦ Synopsis

Plasmid pPGH1 originating from Pseudomonas putida strain H carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. Besides mobilization of pPGH1 by a plasmid of the incompatibility group P-1, hybrid plasmids conferring the Phl+ phenotype could be selected, when R68.45 was the conjugative plasmid. The hybrids contain the complete R68.45 and part of pPGH1. Integration of Phl-DNA of pPGH1 into R68.45 occurred exclusively via the IS21 region of R68.45.