In Vivo Gene Transfer to Mouse Spermatogenic Cells by Deoxyribonucleic Acid Injection into Seminiferous Tubules and Subsequent Electroporation

Efficient gene delivery into murine ovar

Efficient gene delivery into murine ovarian cells by intraovarian injection of plasmid DNA and subsequent in vivo electroporation

✍ Masahiro Sato; Maya Tanigawa; Natsuko Kikuchi; Shingo Nakamura; Minoru Kimura 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 396 KB

## Abstract Summary: We describe the use of direct injection of circular plasmid DNA and subsequent in vivo electroporation (EP) for efficient gene delivery to the ovarian cells, including follicular cells and oocytes of mice. When Trypan blue (TB) was injected into the central portion of an ovary