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In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

✍ Scribed by R. Narayanan; M.M. Jastreboff; C.F. Chiu; J.R. Bertino


Book ID
117056971
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
888 KB
Volume
141
Category
Article
ISSN
0006-291X

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We developed a new in vivo electroporation method to deliver genes into retinal ganglion cells (RGCs). Efficiency and degree of tissue damage were evaluated using green fluorescent protein (GFP) gene and TUNEL. Soon after the intravitreous injection of the GFP gene, electroporation (five electric pu