In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA
โ Scribed by Alexander V. Titomirov; Sergey Sukharev; Elena Kistanova
- Book ID
- 119120097
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 298 KB
- Volume
- 1088
- Category
- Article
- ISSN
- 0167-4781
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## Abstract Summary: We describe the use of direct injection of circular plasmid DNA and subsequent in vivo electroporation (EP) for efficient gene delivery to the ovarian cells, including follicular cells and oocytes of mice. When Trypan blue (TB) was injected into the central portion of an ovary
To examine the effects of inflammatory response to plasmid DNA (pDNA) on transgene expression, serum tumor necrosis factor-a (TNF-a) was measured after intravenous injection of pDNA or calf thymus DNA (CT DNA) in the naked or complexed form with cationic liposomes (lipoplex). pDNA with many CpG moti