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In vivo effects of activated H-ras oncogene expressed in the liver and in urogenital tissues

✍ Scribed by Emmanuelle Gilbert; Arnaud Morel; Micheline Tulliez; Roger Maunoury; Fabiola Terzi; Lucile Miquerol; Axel Kahn

Publisher
John Wiley and Sons
Year
1997
Tongue
French
Weight
243 KB
Volume
73
Category
Article
ISSN
0020-7136

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✦ Synopsis

MATERIAL AND METHODS

Construction of the L-PK/H-ras transgene

A 4.9-kb BamHI fragment containing the 4 exons of the human H-ras gene was purified from the Wap-ras vector (Tremblay et al., 1989) and sub-cloned, at the BglII site, into the vector containing the L-PK gene promoter (extending from position Οͺ3200 in the 5Ј Abbreviations: AST, aspartate aminotransferase; ALT, alanine aminotransferase.

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