In vivo DNA gene electro-transfer: a systematic analysis of different electrical parameters
✍ Scribed by Immacolata Zampaglione; Mirko Arcuri; Manuela Cappelletti; Gennaro Ciliberto; Gemma Perretta; Alfredo Nicosia; Nicola La Monica; Elena Fattori
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 200 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1099-498X
- DOI
- 10.1002/jgm.774
No coin nor oath required. For personal study only.
✦ Synopsis
Background Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes.
Methods
In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short-and long-term protein production.
Results
Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys.