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In Vivo Detection of the Cyclic Osmoregulated Periplasmic Glucan of Ralstonia solanacearum by High-Resolution Magic Angle Spinning NMR

✍ Scribed by J.-M. Wieruszeski; A. Bohin; J.-P. Bohin; G. Lippens

Book ID: 102596383
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 132 KB
Volume: 151
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.2001.2348

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✦ Synopsis

We investigate the mobility of the osmoregulated periplasmic glucans of Ralstonia solanacearum in the bacterial periplasm through the use of high-resolution (HR) NMR spectroscopy under static and magic angle spinning (MAS) conditions. Because the nature of periplasm is far from an isotropic aqueous solution, the molecules could be freely diffusing or rather associated to a periplasmic protein, a membrane protein, a lipid, or the peptidoglycan. HR MAS NMR spectroscopy leads to more reproducible results and allows the in vivo detection and characterization of the complex molecule.

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