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In vivo characterization of several rodent glioma models by 1H MRS

✍ Scribed by Sabrina Doblas; Ting He; Debra Saunders; Jessica Hoyle; Nataliya Smith; Quentin Pye; Megan Lerner; Randy L. Jensen; Rheal A. Towner

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
407 KB
Volume
25
Category
Article
ISSN
0952-3480

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✦ Synopsis

The assessment of metabolites by ^1^H MRS can provide information regarding glioma growth, and may be able to distinguish between different glioma models. Rat C6, 9 L/LacZ, F98 and RG2, and mouse GL261, cells were intracerebrally implanted into the respective rodents, and human U87 MG cells were implanted into athymic rats. Ethyl‐nitrosourea induction was also used. Glioma metabolites [e.g. total choline (tCho), total creatine (tCr), N‐acetylaspartate (NAA), lactate (Lac), glutamine (Gln), glutamate (Glu), aspartate (Asp), guanosine (Gua), mobile lipids and macromolecules (MMs)] were assessed from ^1^H MRS using point‐resolved spectroscopy (PRESS) [TE = 24 ms; TR = 2500 ms; variable pulse power and optimized relaxation delay (VAPOR) water suppression; 27‐μL and 8‐μL voxels in rats and mice, respectively] at 7 T. Alterations in metabolites (Totally Automatic Robust Quantitation in NMR, TARQUIN) in tumors were characterized by increases in lipids (Lip1.3: 8.8–54.5 m m for C6 and GL261) and decreases in NAA (1.3–2.0 m m for RG2, GL261 and C6) and tCr (0.8–4.0 m m for F98, RG2, GL261 and C6) in some models. F98, RG2, GL261 and C6 models all showed significantly decreased (p < 0.05) tCr, and RG2, GL261 and C6 models all exhibited significantly decreased (p < 0.05) NAA. The RG2 model showed significantly decreased (p < 0.05) Gln and Glu, the C6 model significantly decreased (p < 0.05) Asp, and the F98 and U87 models significantly decreased (p < 0.05) Gua, compared with controls. The GL261 model showed the greatest alterations in metabolites. ^1^H MRS was able to differentiate the metabolic profiles in many of the seven rodent glioma models assessed. These models are considered to resemble certain characteristics of human glioblastomas, and this study may be helpful in selecting appropriate models. Copyright © 2011 John Wiley & Sons, Ltd.

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