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In vivo bioluminescent imaging of Schwann cells in a poly(DL-lactide-ε-caprolactone) nerve guide

✍ Scribed by Ming-San Ma; Gooitzen Van Dam; Marcel Meek; Erik Boddeke; Sjef Copray

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
264 KB
Volume
40
Category
Article
ISSN
0148-639X

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✦ Synopsis

Abstract

Nerve guides seeded with Schwann cells (SCs) promote axonal regeneration in peripheral nerve lesions. We examined the applicability of bioluminescent imaging (BLI) for monitoring the fate of SCs in nerve guides after implantation. Rat SCs were transfected with the firefly luciferase (Fluc) gene and subsequently seeded in nerve guides, which were implanted subcutaneously in rats. In vivo bioluminescence of transfected SCs (Fluc‐SCs) was assessed with a BLI system. Scans were validated ex vivo using immunocytochemistry and electron microscopy. We found that BLI enables longitudinal in vivo monitoring of Fluc‐SCs, given that proper access of luciferin to the cells is assured. Muscle Nerve, 2009

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