𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In vivo bioluminescence: A cellular reporter for research and industry

✍ Scribed by Jassim, S. A. A. ;Ellison, A. ;Denyer, S. P. ;Stewart, G. S. A. B.

Publisher
John Wiley and Sons
Year
1990
Weight
491 KB
Volume
5
Category
Article
ISSN
0884-3996

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The detection of specific bacterial pathogens, indicator microorganisms and antimicrobial substances, and the recovery of microorganisms from sub‐lethal injury, are all aspects of importance to industry which are currently being targeted using in vivo bioluminescence. In all instances, a key requirement for the application of bioluminescence is the establishment of a strict correlation between in vivo bioluminescence and cell viability, as determined by colony counting on agar plates. Comparative studies for biocides (phenol, chlorhexidine diacetate, phenol thioether), for a virucide (hypochlorite) and for cellular recovery of S. typhimurium from sub‐lethal injury, all indicate that such a correlation is valid. Furthermore, real‐time measurements of in vivo bioluminescence reveal a major population of bacterial cells that retain functional intracellular biochemistry, but are defective in their ability to replicate post of freeze injury.

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