In vivo assessment of tumor-induced nonspecific suppression of contact sensitivity

✍ Scribed by Ronald E. Garner; Adrien P. Malick; Kevin M. Connolly; Klaus D. Elgert

• Book ID: 104663718
• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 313 KB
• Volume: 18
• Category: Article
• ISSN: 0340-7004
• DOI: 10.1007/bf00205748

✦ Synopsis

Normal BALB/c mice were assessed for 2,4-dinitrofluorobenzene (DNFB)-induced contact sensitivity following adoptive transfer of macrophages (Mo). T cells, or their derived products, from normal or tumor-bearing hosts (TBH). Contact sensitivity (CS) was measured by a quantitative radioisotopic ear assay, a total in vivo system based on localization of IP-injected iodinated human serum albumin [( 125I]HSA) in the DNFB-challenged ear. Adoptive transfer of low or high doses of TBH T cells or their derived supernatants into normal recipients suppressed their responsiveness, while Mo supernatants enhanced it. Moreover, in all cases adoptive transfer of TBH cells or supernatants resulted in a lower CS response than did their normal counterparts. These results further corroborate our previous in vitro data indicating that T cells, or Mo and T cell soluble products, possess immunoregulatory capabilities in vivo.