In vivo antibacterial and silver-releasing properties of novel thermal sprayed silver-containing hydroxyapatite coating

✍ Scribed by Takafumi Shimazaki; Hiroshi Miyamoto; Yoshiki Ando; Iwao Noda; Yutaka Yonekura; Shunsuke Kawano; Masaki Miyazaki; Masaaki Mawatari; Takao Hotokebuchi

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 160 KB
• Volume: 9999B
• Category: Article
• ISSN: 1552-4973
• DOI: 10.1002/jbm.b.31526

✦ Synopsis

Abstract

One of the serious postoperative complications associated with joint replacement is bacterial infection. In addressing this problem, we have previously described the development of a novel thermal spraying technology combining silver (Ag) showing antibacterial activity with hydroxyapatite (HA) displaying good biocompatibility and osteoconductivity, and reported the in vitro properties. This study evaluated serum Ag ion concentrations and antibacterial activity against methicillin‐resistant Staphylococcus aureus (MRSA) using a subcutaneous rat model. HA loaded with 3 wt % of silver oxide (Ag‐HA) and plain HA were sprayed on the surface of titanium disks. Ag‐HA‐ or HA‐coated samples were implanted into the back subcutaneous pockets of male Sprague‐Dawley rats. Mean serum Ag ion concentration in the Ag‐HA group increased to more than 50 ppb by 48 h after implantation, then decreased gradually to baseline levels. Mean (± standard error of the mean) number of viable MRSA on HA coating was (1.5 ± 0.5) × 10^5^, which is significantly more than the (1.1 ± 0.4) × 10^4^ on Ag‐HA coating (p < 0.001). Ag‐HA coating offers good abilities to release Ag ions and kill MRSA in vivo. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010