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In vivo and in vitro protein solubility assays using split GFP

✍ Scribed by Cabantous, Stéphanie; Waldo, Geoffrey S

Book ID
109938018
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
349 KB
Volume
3
Category
Article
ISSN
1552-4450

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In vivo and in vitro protein solubility
In vivo and in vitro protein solubility assays using split GFP
✍ Cabantous, Stéphanie; Waldo, Geoffrey S 📂 Article 📅 2006 🏛 Nature Publishing Group 🌐 English ⚖ 349 KB

The rapid assessment of protein solubility is essential for evaluating expressed proteins and protein variants for use as reagents for downstream studies. Solubility screens based on antibody blots are complex and have limited screening capacity 1-3 . Protein solubility screens using split β-galacto