In vivo and in vitro association of 14–3-3 epsilon isoform with calmodulin: Implication for signal transduction and cell proliferation

✍ Scribed by Sharon C.W. Luk; Sai-ming Ngai; Stephen K.W. Tsui; Kwok-pui Fung; Cheuk-yu Lee; Mary M.Y. Waye

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 59 KB
• Volume: 73
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/(sici)1097-4644(19990401)73:1<31::aid-jcb4>3.0.co;2-x

✦ Synopsis

Using a yeast two-hybrid screen, human 14-3-3 epsilon protein was found to interact with human calmodulin. In vitro binding assay between human 14-3-3 epsilon protein/peptide and calmodulin was demonstrated by native gel electrophoresis, and the interaction was shown to be calcium dependent. Our results, along with the association of the 14-3-3 epsilon protein with other signaling proteins, suggest that the 14-3-3 protein could provide a link between signal transduction and cell proliferation.